The hospital believes no more can be done for Alfie and has applied to withdraw treatment, but his parents Tom Evans and Kate James have argued passionately that they should be allowed to move their son to another specialist children's hospital in Italy.
Alfie was born on 9 May 2016.
A spokeswoman for the Christian Legal Centre, which is assisting them, said an air ambulance was "waiting outside Alder Hey Hospital ready to take Alfie to hospital in Italy".
A statement from the hospital says: "The High Court again ruled that it is in Alfie's best interests to continue with the end of life care plan developed by the clinical team who have cared for him throughout".
Adding insult to injury, Alfie's father claims that his son has been denied nutrition for 23 hours, suggesting that now that the boy is breathing on his own, the hospital is attempting to starve him.
He has been in a "semi-vegetative state" for more than a year and is now only receiving oxygen after being taken off life support last night.
Dad Tom Evans tells the ECHO he fears Alder Hey could mount a legal battle to switch Alfie's life support off, as it is not clear Alfie can recover. "All my support to Alfie and his extraordinary parents".
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Charlie's parents turn to Dr Michio Hirano, the United States neurologist who offered to provide revolutionary treatment to help Charlie Gard. The judge, a gay-rights activist named Sir Anthony Paul Hayden, the author of Children and Same Sex Families: A Legal Handbook and a member of The Bar Lesbian and Gay Group (BLAGG), rejected Alfie's parents' pleas that they know better that the courts where their child's best interests lie.
The parents of seriously ill toddler whose been at the centre of a life support treatment battle will challenge a High Court ruling preventing them from taking him to Italy for further treatment.
Instead, Evans will remain at Alder Hey Children's Hospital, or may eventually be permitted to return home with his parents.
Hayden said during Tuesday's hearing that the best scenario for Alfie's parents was to look into the possibility of transferring him from intensive care to a hospice, ward or his home, according to the BBC.
The hospital's doctors and independent medical experts say there is no cure and no hope for Alfie.
The hospital said it would not be offering a running commentary on the child's condition.
Police are having to block protestors from storming the hospital.
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"So now we see what the three judges in the Court of Appeal can do to us", he said.
In delivering the news, they said their case was 'inadmissable'.
On Monday, the Italian foreign ministry announced it had granted Alfie Italian citizenship to facilitate his arrival and transport. "Whatever happens with Alfie there will be other children for sure that the state will want to kill", he continued.
As time went by, the doctors at Alder Hey Children's Hospital staff have not been able to give a diagnosis of what is wrong with Alfie.
Mr Justice Hayden, speaking after an emergency hearing in the Family Division of the High Court in Manchester, called Alfie a "warrior", but said the case had reached its "final chapter".
Monday, with the support of the United Kingdom court system, Alder Hey removed life support from Alfie.
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